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Thursday, July 16, 2020 | History

2 edition of enumeration of bacteriophages in water and sewage found in the catalog.

enumeration of bacteriophages in water and sewage

Ann H. Vajdic

enumeration of bacteriophages in water and sewage

by Ann H. Vajdic

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  • 9 Currently reading

Published by Ontario Water Resources Commission in [Toronto?] .
Written in English

    Subjects:
  • Bacteriophages.

  • Edition Notes

    SeriesDivision of Research paper : -- .no.2010, Paper (Ontario Water Resources Commission. Division of Research) -- no.2010
    The Physical Object
    Pagination9 p. :
    ID Numbers
    Open LibraryOL16886780M

    4. Measurement of the attachments rate of bacteriophage to bacterial cell. Experimental Samples Collection: Water Samples: One hundred of sewage water samples were collected from different environmental sources in a sterile method in clean containers, from this samples ten nosa were isolated and used as a hosts for isolation of phages.   The Microbiology of Drinking Water () - Part 4 - Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli OH7) Ref: Blue Book .

    Isolation of Bacteriophage from Sewage and Determination of Phage TiterEXERCISE 37 (a) (b) Bacteriophage Plaque Figure Phage plaques.(a) A lawn of E. coliB containing plaques.(b. Viruses and phages in water and the solid compartments undergo inactivation by both natural and human-introduced physical, chemical, and biological stressors. In primary sedimentation, flocculation-aided sedimentation, activated sludge digestion, activated sludge digestion plus precipitation, and upflow anaerobic sludge blanket processes, the die-off of the microorganisms seems to play a minor.

    and TC levels in water can be detected and enumerated. Summary of Method. An appropriate volume of a water sample ( mL for drinking water) is filtered through a mm, µm pore size cellulose ester membrane filter that retains the bacteria present in the sample. Two groups of bacteriophages that infect E. coli, somatic and F-specific coliphages, have been used as both fecal and viral indicators in academia for many years [1,2,3,4].The high concentration of coliphages found in raw wastewater and many other matrices contaminated with fecal remains, the easy, fast, and cost-effective detection and enumeration methods, their persistence in the water.


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Enumeration of bacteriophages in water and sewage by Ann H. Vajdic Download PDF EPUB FB2

Bacteriophage populations in an activated-sludge sewage treatment plant were enumerated. A newly developed assay for quantitation of total phages, employing direct electron microscopic counts, was used in conjunction with the plaque by:   Among these approaches, the use of model bacteriophages as surrogates to monitor membrane integrity has been considered as an online indirect test because (1) human virus seeding is hardly feasible or safe in water and wastewater treatment and (2) naturally occurring bacteriophages are thought to be the most suitable surrogates for enteric Cited by: The book presents studies that deal primarily with the virological aspect of water sanitation.

The topics covered in the selection include methods for studying virus in waste waters, such as virus recovery from wastewater using microfiberglass filters and rapid detection of enteroviruses in water by a quantitative fluorescent antibody technique.

the aim of this study was to isolate bacteriophage from sewage water of udsm oxidation ponds capable of lysing enteric And plaques was the only way to determine if the project has been successfull.

Bacteriophages infecting Bacteroides fragilis GB have been described as potential markers of human fecal contamination in water sources. The aim of this study was to evaluate the occurrence of GB phages in raw sewage, secondary effluents and reclaimed water of the São Paulo city using a low-cost microbial source tracking method.

Bacteriophages are present in ecosystems which support bacterial multiplication, such as thebovine(2, 19) andsheep(13) rumens,natural waters (4), soil (10), cheese-makingvats (4), and feces and sewage (4, 11, ).

However, few studies have beenmadeofthe quantitative re-lationship of both phages and host bacteria in these systems. Bacteriophage present an ideal system for development of curricular exercises that use the plaque assay because their host cells are easier and safer to grow in the laboratory than animal cells.

enumeration of bacteriophages in water and sewage book In this laboratory activity students use a plaque assay to detect the presence of bacteriophage in a natural environmental sample—human sewage effluent.

Isolated bacteriophages could be used potentially as a biocontrol agent for foaming problems in activated sludge treatment plants. Potential application of bacteriophage need much attention for the control of foaming as it is the ecofriendly and cost effective approach.

Phage Enumeration Material: 1. Recipient E. coli broth culture 2. P1 bacteriophage transducing lysate (10 l) 3. Six preheated LB plates 4.

Six 3 ml tubes of H top agar 5. Sterile saline and 13 microfuge tubes for dilutions 6. Preheated (37˚C & 60˚C) water baths Protocol: (working in pairs) 1. The CDC indicates that “there is no evidence that the virus that causes COVID can be spread to people through the water in pools, hot tubs, spas, or water play areas.

The bacterial host strains used for enumeration of the bacteriophages were: WG5 E. coli (ATCC ) for SOMCPH; WG49 Salmonella Typhimurium (NCTC or ATCC ) for FRNAPH and HSP40 B.

fragilis (ATCC ) for BFRPH. Theodora Ijeoma Ekwomadu, Nomathamsanqa Patricia Sithebe, Enumeration of Bacteriophages from Mmabatho Treatment Plant and Some Selected Water Sources in Ngaka Modiri Molema District of North-West Province, South Africa, International Journal of Microbiology and Biotechnology.

Vol. 2, No. 2,pp. doi: /   Bacteriophages, coliform and fecal coliform bacteria in wastewater in southern Thailand P. Claydong, S.

Danteravanich, C. Siriwong and S. Uakritdathikarn Faculty of Environmental Management, Prince of Songkla University, P.O.

Kho Hong, Hat Yai, SongkhlaThailand The study of microbial communities in wastewater has generally focused on water pollution and water. Isolation of Potential Bacterial Enteric Host Strains from Activated Sewage Sludge Two containers (2 liters) of sewage sludge were obtained from the waste water treatment facility located in Nashua, NH.

Bacteria were isolated from the sewage sludge by plating ml of sludge onto either TS or TSBW agar. In this investigation 49 virus-host systems were studied by isolating co-occurring bacteria and bacteriophages from the aeration basin of a sewage treatment plant during 5 successive weeks.

A method for the enumeration of male-specific bacteriophages in sewage. J Appl Bacteriol ; 5. IAWPRC, Study Group On Water virology. The health significance of viruses in water. Water Research ; 6. Mossel DAA. Index and indicator organisms a current assessment of their usefulness and significance.

The results of the comparison of the two host strains for the enumeration of the F-specific bacteriophages are given in Fig. 1a and b. In Fig. 1a the data are given for the total number of F-specific bacteriophages (FTOTPH).

In Fig. 1b the number of F-specific RNA phages (FRNAPH) found with both host strains is given. In the two figures it is shown that the results for both host strains are. In the presence of bacteriophages, plaques could be enumerated after an overnight exposure of the concentrate to the host bacterial strains WG49 and WG5.

The bacteriophage concentration in the original water sample was calculated from the tested volume and the phage counts.

F specific bacteriophages were detected in all sewage samples investigated in numbers ranging from 10 2 − 5 × 10 4 pfu/ml. Data are presented on the number and types of F specific phages in mixed domestic/industrial sewage; in poultry processing wastewater and in hospital wastewater.

The potential of F specific phages as water quality. Microbial source tracking methods are designed to enable researchers to uncover the sources of fecal pollution in a water body ().Bacteriophages infecting Bacteroides are potential tools for microbial source tracking (4, 13, 22, 24, 26, 29).However, it is well documented that Bacteroides host strains vary in their ability to discriminate between phages of different sources but also that phage.

Abstract. Here we describe the detection, enumeration, and isolation of bacteriophages infecting method is based on the infection of Bacteroides host strains and the production of visible plaques in a confluent lawn of the host strain using the double-layer agar method.

This is a straightforward methodology that can be applied for the detection, enumeration and isolation of. Waterborne bacterial pathogens in wastewater remains an important public health concern, not only because of the environmental damage, morbidity and mortality that they cause, but also due to the high cost of disinfecting wastewater by using physical and chemical methods in treatment plants.

Bacteriophages are proposed as bacterial pathogen indicators and as an alternative .water quality - general guidance on the enumeration of micro-organisms by culture: iso (r) water quality - detection and enumeration of bacteriophages - part 4: enumeration of bacteriophages infecting bacteroides fragilis: iso tr water quality - guidance on validation of microbiological methods: iso